Microabscesses of the liver and spleen were observed in six leukemic patients after chemotherapy. Clinical symptoms included fever, abdominal pain, and hepatic dysfunction. Ultrasonography (US) disclosed multiple small hypoechoic lesions with a bull's eye appearance in the liver. Bacteriological diagnosis was performed in only one case, in which Candida tropicalis was cultured from the blood. The other cases were diagnosed by imaging studies using US and computed tomography (CT), as well as according to symptoms. US findings are not specific, but fungal microabscesses of the liver and spleen were characteristic after chemotherapy in leukemic patients. Because of its sensitivity, US is useful in the early detection of microabscesses, as well as for follow-up studies.